Journal: Annals of Translational Medicine

Article Title: Cornel iridoid glycoside ameliorates cognitive deficits in APP/PS1/tau triple transgenic mice by attenuating amyloid-beta, tau hyperphosphorylation and neurotrophic dysfunction

doi: 10.21037/atm.2020.02.138

Figure Lengend Snippet: The effects of CIG on activity-related modifications of PP2A and GSK-3β in the cerebral cortex and hippocampus of 3×Tg mice (western blotting). (A) Representative blots of PP2A catalytic subunit C (PP2Ac) methylated PP2Ac at Leu309 (m-PP2Ac), GSK-3β and phosphorylated GSK-3β at Ser9 (p-GSK3β-Ser9). Quantitative findings of PP2Ac, m-PP2Ac, GSK-3β and p-GSK3β-Ser9 in cerebral cortex (B) and hippocampus (C). Internal control was β-actin. Ratio of target protein to β-actin in WT control mice was set as 1. Data are expressed as means ± SEM; n=4 per group. ###, P<0.001, 3×Tg group vs. WT group; *, P<0.05, **, P<0.01, ***, P<0.001, CIG-treated groups vs. 3×Tg group. CIG, cornel iridoid glycoside.

Article Snippet: The monoclonal primary antibodies used in these assays comprised rabbit anti-Aβ antibody diluted 1:1,000; mouse anti-CREB antibody diluted 1:1,000 and rabbit anti-phosphorylated CREB antibody diluted 1:1,000 (Cell Signaling Technology), rabbit anti-APP antibody diluted 1:500, rabbit anti-BACE1 antibody diluted 1:10,000, rabbit anti-IDE antibody diluted 1:10,000, rabbit anti-p-GSK3β-Ser9 antibody diluted 1:1,000 and rabbit anti-NGF antibody diluted 1:1,000 (Abcam, Cambridge, UK); rabbit anti-ADAM10 antibody (diluted 1:10,000; Sigma), rabbit anti-NEP antibody diluted 1:1,000 (Merck-Millipore); rabbit anti-PP2Ac antibody diluted 1:500 and mouse anti-methylated PP2Ac antibody diluted 1:100 (Santa Cruz Biotechnology Inc., Dallas TX, USA); rabbit anti-GSK-3β antibody diluted 1:1,000 and mouse anti-β-actin antibody diluted 1:1,000 (ZSGB-Biotechnology Company, Beijing, China); rabbit anti-BDNF antibody diluted 1:2,000 (Epitomics International Inc., Burlingame, CA, USA); rabbit anti-phosphorylated tau at Ser396 antibody diluted 1:1,000 and rabbit anti-phosphorylation tau at Ser44 site antibody diluted 1:1,000 (Invitrogen, Waltham, MA, USA).

Techniques: Activity Assay, Western Blot, Methylation, Control

Journal: Horticulture Research

Article Title: ALA reverses ABA-induced stomatal closure by modulating PP2AC and SnRK2.6 activity in apple leaves

doi: 10.1093/hr/uhad067

Figure Lengend Snippet: Western blotting assay of phosphorylated MdSnRK2.6 and MdPP2AC protein abundances in OE- MdPP2AC apple leaves treated with exogenous ALA and/or ABA . A : Western blotting assay. B and C : Quantification of grey levels of the phosphorylated SnRK2.6 and PP2AC protein bands. Proteins were extracted from the apple leaf epidermis under different treatments before immunoprecipitation. Molecular mass (kDa) markers are shown on the left. All experiments were repeated at least three times with similar results. The same lowercase letters indicate no significant difference at p = 0.05

Article Snippet: The blots were probed with a customized anti-MdPP2AC peptide-raised (SHSDLDRQIEHLMEC) antibody to MdPP2AC (Abclonal Biotechnology Co., Ltd.) ( ) and a phosphorylated anti-PP2AC antibody (Abclonal Biotechnology Co., Ltd.; Catalog No. AP1043) ( ).

Techniques: Western Blot, Immunoprecipitation

Journal: Frontiers in Oncology

Article Title: PPP2CA knockdown upregulates the expression levels of ferroptosis-related genes TFRC and ACSL4 in colorectal cancer cells by promoting mTOR phosphorylation

doi: 10.3389/fonc.2025.1694932

Figure Lengend Snippet: The expression levels of PP2Acα, p-mTOR, Acsl4, and TfR in HCT116 CRC cell lines (A) Protein electrophoresis image; (B-E) Protein quantification images of PP2Acα, p-mTOR, Acsl4, and TfR, respectively * P < 0.05, ** P < 0.01. n=3, Mean ± SD.

Article Snippet: The anti-PP2AC antibody (cat. no. 13482-1-AP) and anti-GAPDH antibody (cat. no. 10494-1-AP) were acquired from Wuhan Sanying Biotechnology Co., Ltd. Additionally, the anti-mTOR (phospho Ser2448) antibody (cat. no. YP-Ab-14329), anti-ACSL4 antibody (cat. no. YP-Ab-12589), and anti-TfR antibody (cat. no. YP-Ab-14016) were purchased from Hangzhou Zhenyoupin Biotechnology Co., Ltd. Rapamycin (cat. no. R485503) was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd.

Techniques: Expressing, Protein Electrophoresis